Inozin monofosfatna sintaza

5-Aminoimidazol-4-karboksamid ribonukleotid formiltransferaza/IMP ciklohidrolaza

PDB prikaz baziran na 1p4r

Dostupne strukture

1P4R, 1PKX, 1PL0

Identifikatori

Simboli

ATIC; AICAR; AICARFT; IMPCHASE; PURH

Vanjski ID

OMIM: 601731 MGI: 1351352 HomoloGene: 2983 GeneCards: ATIC Gene

EC broj

3.5.4.10 2.1.2.3, 3.5.4.10

Ontologija gena
Molekularna funkcija	• IMP ciklohidrolazna aktivnost • fosForibozilaminoimidazolkarboksamid formiltransferazna aktivnost • proteinska homodimerizaciona aktivnost
Celularna komponenta	• mitohondrija • citozol
Biološki proces	• nujleobazni metabolički proces • purinski nukleobazni metabolički proces • 'de novo' IMP biosintetički proces

Pregled RNK izražavanja

podaci

Ortolozi

Vrsta

Čovek

Miš

Entrez

471

108147

Ensembl

ENSG00000138363

ENSMUSG00000026192

UniProt

P31939

Q9CWJ9

RefSeq (mRNA)

NM_004044

NM_026195

RefSeq (protein)

NP_004035

NP_080471

Lokacija (UCSC)

Chr 2:
216.18 - 216.21 Mb

Chr 1:
71.56 - 71.58 Mb

PubMed pretraga

[1]

[2]

Bifunkcionalni purinski biosintetički protein PURH je protein koji je kod čoveka kodiran ATIC genom.^[1]^[2]^[3]

ATIC kodira enzim koji generiše inozin monofosfat iz aminoimidazol karboksamid ribonukleotida.

On ima dve funkcije:

EC 2.1.2.3 - 5-aminoimidazol-4-karboksamid ribonukleotid formiltransferaza
EC 3.5.4.10 - IMP ciklohidrolaza

Reference

^ Rayl EA, Moroson BA, Beardsley GP (1996). „The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. Cloning, sequencing, expression, purification, kinetic analysis, and domain mapping”. J Biol Chem. 271 (4): 2225—33. PMID 8567683. doi:10.1074/jbc.271.4.2225.
^ Sugita T, Aya H, Ueno M, Ishizuka T, Kawashima K (1997). „Characterization of molecularly cloned human 5-aminoimidazole-4-carboxamide ribonucleotide transformylase”. J Biochem. 122 (2): 309—13. PMID 9378707.
^ „Entrez Gene: ATIC 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase”.

Literatura

Rasmussen HH; van Damme J; Puype M; et al. (1993). „Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes”. Electrophoresis. 13 (12): 960—9. PMID 1286667. doi:10.1002/elps.11501301199.
Yamauchi M; Seki N; Mita K; et al. (1996). „Isolation of human purH gene expressed in the rodent transformant cells by subtractive enrichment of 3'-untranslated region of human transcript”. DNA Res. 2 (6): 269—75. PMID 8867801. doi:10.1093/dnares/2.6.269.
Beardsley GP; Rayl EA; Gunn K; et al. (1998). „Structure and functional relationships in human pur H”. Adv. Exp. Med. Biol. Advances in Experimental Medicine and Biology. 431: 221—6. ISBN 978-0-306-45778-4. PMID 9598063. doi:10.1007/978-1-4615-5381-6_43.
Vergis JM, Bulock KG, Fleming KG, Beardsley GP (2001). „Human 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase. A bifunctional protein requiring dimerization for transformylase activity but not for cyclohydrolase activity”. J. Biol. Chem. 276 (11): 7727—33. PMID 11096114. doi:10.1074/jbc.M009940200.
Bulock KG, Beardsley GP, Anderson KS (2002). „The kinetic mechanism of the human bifunctional enzyme ATIC (5-amino-4-imidazolecarboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase). A surprising lack of substrate channeling”. J. Biol. Chem. 277 (25): 22168—74. PMID 11948179. doi:10.1074/jbc.M111964200.
Strausberg RL; Feingold EA; Grouse LH; et al. (2003). „Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences”. Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899—903. PMC 139241 . PMID 12477932. doi:10.1073/pnas.242603899.
Cheong CG; Wolan DW; Greasley SE; et al. (2004). „Crystal structures of human bifunctional enzyme aminoimidazole-4-carboxamide ribonucleotide transformylase/IMP cyclohydrolase in complex with potent sulfonyl-containing antifolates”. J. Biol. Chem. 279 (17): 18034—45. PMID 14966129. doi:10.1074/jbc.M313691200.
Marie S; Heron B; Bitoun P; et al. (2004). „AICA-Ribosiduria: A Novel, Neurologically Devastating Inborn Error of Purine Biosynthesis Caused by Mutation of ATIC”. Am. J. Hum. Genet. 74 (6): 1276—81. PMC 1182092 . PMID 15114530. doi:10.1086/421475.
Dervieux T; Furst D; Lein DO; et al. (2004). „Polyglutamation of methotrexate with common polymorphisms in reduced folate carrier, aminoimidazole carboxamide ribonucleotide transformylase, and thymidylate synthase are associated with methotrexate effects in rheumatoid arthritis”. Arthritis Rheum. 50 (9): 2766—74. PMID 15457444. doi:10.1002/art.20460.
Gerhard DS; Wagner L; Feingold EA; et al. (2004). „The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)”. Genome Res. 14 (10B): 2121—7. PMC 528928 . PMID 15489334. doi:10.1101/gr.2596504.
Ewing RM; Chu P; Elisma F; et al. (2007). „Large-scale mapping of human protein–protein interactions by mass spectrometry”. Mol. Syst. Biol. 3 (1): 89. PMC 1847948 . PMID 17353931. doi:10.1038/msb4100134.

PDB Galerija

1p4r: Kristalna struktura ljudskog ATIC u kompleksu sa folat-baziranim inhibitorom BW1540U88UD
1pkx: Kristalna struktura ljudskog ATIC u kompleksu sa XMP
1pl0: Kristalna struktura ljudskog ATIC u kompleksu sa folat-baziranim inhibitorom, BW2315U89UC