Prostaglandin-E2 9-reductase

In enzymology, a prostaglandin-E₂ 9-reductase (EC 1.1.1.189) is an enzyme that catalyzes the chemical reaction

(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP⁺ ${\displaystyle \rightleftharpoons }$ (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NADPH + H⁺

Thus, the two substrates of this enzyme are (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate and NADP⁺, whereas its 3 products are (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate:NADP⁺ 9-oxidoreductase. Other names in common use include PGE₂-9-OR, reductase, 15-hydroxy-9-oxoprostaglandin, 9-keto-prostaglandin E₂ reductase, 9-ketoprostaglandin reductase, PGE-9-ketoreductase, PGE₂ 9-oxoreductase, PGE₂ reductase-9-ketoreductase, prostaglandin 9-ketoreductase, prostaglandin E 9-ketoreductase, and prostaglandin E₂ reductase-9-oxoreductase. This enzyme participates in arachidonic acid metabolism.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1Q13, 1Q5M, and 2PFG.

References

• Lee SC, Levine L (1975). "Purification and regulatory properties of chicken heart prostaglandin E 9-ketoreductase". J. Biol. Chem. 250 (12): 4549–55. PMID 166995.
• Schlegel W, Kruger S, Korte K (1984). "Purification of prostaglandin E₂ reductase-9-oxoreductase from human decidua vera". FEBS Lett. 171 (1): 141–4. doi:10.1016/0014-5793(84)80475-5. PMID 6586494. S2CID 25623206.
• Tai HH, Yuan B (1982). "Purification and assay of 9-hydroxyprostaglandin dehydrogenase from rat kidney". Methods Enzymol. 86: 113–7. doi:10.1016/0076-6879(82)86178-8. PMID 7132747.
• Watkins JD, Jarabak J (1985). "The effect of NaCl intake on 9-ketoprostaglandin reductase activity in the rabbit kidney". Prostaglandins. 30 (2): 335–49. doi:10.1016/0090-6980(85)90196-0. PMID 3901124.