Middlebrook 7H10 Agar

Growth medium used to culture Mycobacterium species

Middlebrook 7H10 Agar is a solid growth medium specially used for culture of Mycobacterium, notably Mycobacterium tuberculosis.^[1] It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.

Composition

Ingredients (g/L)^[2]

Ammonium sulfate, 0.50
Monopotassium phosphate, 1.50
Disodium phosphate, 1.50
Sodium citrate, 0.40
Magnesium sulfate, 0.025
Zinc sulfate, 0.001
Copper sulfate, 0.001
L-Glutamic acid, 0.50
Ferric ammonium citrate, 0.04
Pyridoxine hydrochloride, 0.001
Biotin, 0.0005
Malachite green, 0.00025
Agar, 15.00
Calcium chloride, 0.0005

Cultures should be read within 5–7 days after inoculation and once a week thereafter for up to 8 weeks.

References

^ Atlas, Ronald M.; James W. Snyder (2006). Handbook of media for clinical microbiology. CRC Press. p. 307. ISBN 978-0-8493-3795-6.
^ Middlebrook 7H10 Agar Base

External links

Middlebrook 7H10 Agar
Traditional Microbiology

Growth media / agar plates

Selective media

Gram positive

Actinomycetota	Mycobacterium tuberculosis Löwenstein–Jensen medium Middlebrook 7H9 Broth Middlebrook 7H10 Agar Middlebrook 7H11 Agar Mycoplasma pneumoniae Eaton's agar
Bacillota	Corynebacterium diphtheriae Hoyle's agar Enterococcus Bile esculin agar Lactobacillus Lee's Agar MRS agar Lactococcus M17 agar Listeria Fraser broth PALCAM Staphylococcus Mannitol salt agar Baird-Parker agar Vogel–Johnson agar

Gram negative

Alphaproteobacteria	Brucella Brucella agar Farrell's medium
Betaproteobacteria	Neisseria Thayer–Martin agar New York City agar
Gammaproteobacteria	Aggregatibacter actinomycetemcomitans Tryptic soy-serum-bacitracin-vancomycin Bordetella Bordet–Gengou agar Enterobacteriaceae VRBD agar Haemophilus influenzae/Legionella pneumophila Buffered charcoal yeast extract agar Pseudomonas aeruginosa Cetrimide agar Salmonella XLT agar DCA agar Salmonella/Shigella XLD agar